BLAST Help


Available search programs

------------------------- blastn - Comparing a nucleotide query sequence against a nucleotide sequence database. blastp - Comparing an amino acid query sequence against a protein sequence database. blastx - Comparing a nucleotide query sequence translated in all reading frames aginst a protein sequence database.

Parameters

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expect

Specify the expected number of homologous sequences in the database. If you need to get more sequences with lower homology score, increase the "expect" value. If you need only sequences with very high homology scores, decrease the value. [Real] Default value: 10.0

filter

The server filters your query sequence for low compositional complexity regions by default. Low complexity regions commonly give spuriously high scores that reflect compositional bias rather than significant position-by- position alignment. Filtering can elminate these potentially confounding matches (e.g., hits against proline-rich regions or poly-A tails) from the blast reports, leaving regions whose blast statistics reflect the specificity of their pairwise alignment. Queries searched with the blastn program are filtered with DUST. Other programs use SEG. Low complexity sequence found by a filter program is substituted using the letter "N" in nucleotide sequence (e.g., "NNNNNNNNNNNNN") and the letter "X" in protein sequences (e.g., "XXXXXXXXX").

query_code (blastx only)

Specify Query Genetic code to be used in blastx translation of the query. Query genetic code table: 1 = Standard 2 = Vertebrate Mitochondrial 3 = Yeast Mitochondrial 4 = Mold Mitochondrial 5 = Invertebrate Mitochondrial 6 = Ciliate Macronuclear 9 = Echinodermate Mitochondrial 10 = Euplotid Nuclear 11 = Bacterial 12 = Alternative Yeast Nuclear 13 = Ascidian Mitochondrial 14 = Flatworm Mitochondrial 15 = Blepharisma Macronuclear Default value: 1